Article
[Genotype detection of the merozoite surface protein alleles of Plasmodium vivax].
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 30 Apr 2004
Zhang Shan-ying, Xu Long-shan, Lu Hui-min, Zhang Ying-zhen, Gao Qi, Li Li-sha
Abstract excerpt
OBJECTIVE: To develop a method for detecting the genotype of Plasmodium vivax merozoite surface protein 1 (PvMSP-1) alleles. METHODS: According to the sequence characteristic of PvMSP-1, nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to amplify the polymorphic region of ICB5-ICB6 which contains Q repeats and PvuII restriction site (Sal-1 type). The PCR...
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