Characterization and genetic manipulation of peptide synthetases in Pseudomonas aeruginosa PAO1 in order to generate novel pyoverdines.

doi:10.1016/j.chembiol.2004.04.014

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Article

Characterization and genetic manipulation of peptide synthetases in Pseudomonas aeruginosa PAO1 in order to generate novel pyoverdines.

Chemistry & biology - 1 Jul 2004

Ackerley David F, Lamont Iain L

Abstract excerpt

PvdD, a nonribosomal peptide synthetase (NRPS) of Pseudomonas aeruginosa PAO1, incorporates two L-threonines into the siderophore pyoverdine. A pvdD mutant did not synthesize pyoverdine and lacked a high Mr iron-regulated cytoplasmic protein (IRCP). Analysis of other IRCPs and the P. aeruginosa genome enabled the remaining pyoverdine NRPSs to be identified. The pvdD mutation could be complemented in trans,...

Topics

Amino Acid Sequence
Base Sequence
Genetic Complementation Test
Genome, Bacterial
Molecular Sequence Data
Mutation
Oligopeptides
Peptide Synthases
Pseudomonas aeruginosa
Sequence Homology, Amino Acid

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Identifiers

PMID: 15271355
DOI: 10.1016/j.chembiol.2004.04.014

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