Article
Application of a high-throughput HPLC-MS/MS assay to Arabidopsis mutant screening; evidence that threonine aldolase plays a role in seed nutritional quality.
The Plant journal : for cell and molecular biology - 1 Aug 2004
Jander Georg, Norris Susan R, Joshi Vijay, Fraga Michele, Rugg Arthur, Yu Shaoxia, Li Lily, Last Robert L
Abstract excerpt
Beyond their essential function as the building blocks of proteins, amino acids contribute to many aspects of plant biochemistry and physiology. Despite this, there are relatively large gaps in our understanding of the biochemical pathways and regulation of amino acid synthesis in plants. A rapid (1.5 min versus 20-90 min for standard methods) HPLC-MS/MS assay for separating 19 amino acids was developed for...
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