Article
Site-directed mutagenesis provides insight into racemization and transamination of alanine catalyzed by Treponema denticola cystalysin.
The Journal of biological chemistry - 27 Aug 2004
Cellini Barbara, Bertoldi Mariarita, Paiardini Alessandro, D'Aguanno Simona, Voltattorni Carla Borri
Abstract excerpt
In addition to alpha, beta-elimination of L-cysteine, Treponema denticola cystalysin catalyzes the racemization of both enantiomers of alanine accompanied by an overall transamination. Lys-238 and Tyr-123 or a water molecule located on the si and re face of the cofactor, respectively, have been proposed to act as the acid/base catalysts in the proton abstraction/donation at Calpha/C4' of the external aldimine. In...
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