Article
[A new PCR method for direct determination of RHD zygosity].
Zhonghua yi xue za zhi - 2 May 2004
Shao Chao-peng, Xiong Wen
Abstract excerpt
OBJECTIVE: To establish a direct method for the determination of RHD zygosity. METHODS: Two pairs of primers were designed specific for hybrid Rhesus box and exon 1 of RHD, respectively. Combined with a pair of internal control primers, a new dual-tube PCR method was established. One hundred and fifty-two DNA samples, of which the sequence of RHD and RH genotypes were known, and samples from 359 donors or...
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