Article
Sequence typing reveals extensive strain diversity of the Lyme borreliosis agents Borrelia burgdorferi in North America and Borrelia afzelii in Europe.
Microbiology (Reading, England) - 1 Jun 2004
Bunikis Jonas, Garpmo Ulf, Tsao Jean, Berglund Johan, Fish Durland, Barbour Alan G
Abstract excerpt
The genetic polymorphism of Borrelia burgdorferi and Borrelia afzelii, two species that cause Lyme borreliosis, was estimated by sequence typing of four loci: the rrs-rrlA intergenic spacer (IGS) and the outer-membrane-protein gene p66 on the chromosome, and the outer-membrane-protein genes ospA and ospC on plasmids. The major sources of DNA for PCR amplification and sequencing were samples of the B. burgdorferi...
Topics
- Animals
- Base Sequence
- Borrelia burgdorferi
- Borrelia burgdorferi Group
- DNA, Bacterial
- Europe
- Genetic Variation
- Humans
- Ixodes
- Lyme Disease
- Molecular Sequence Data
- North America
- Phylogeny
- Recombination, Genetic