Article
Typing single-nucleotide polymorphisms in Toxoplasma gondii by allele-specific primer extension and microarray detection.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2004
Su Chunlei, Hott Christian, Brownstein Bernard H, Sibley L David
Abstract excerpt
Genotyping is an important tool for epidemiological and population genetic studies in protozoan parasites. The most commonly used method for genotyping is polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) analysis of single nucleotide polymorphisms (SNPs). However, PCR-RFLP analysis is labor intensive, and only a proportion of the SNPs are recognized by currently available...
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