Article
A simple method to generate PCR-RFLP typing profiles from DNA sequences in Toxoplasma gondii.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Nov 2020
Castro Bruno Bello Pede, Gennari Solange Maria, Lorenzi Hernan, Su Chunlei
Abstract excerpt
Multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) has been widely used to genotype microorganisms. Accumulation of data generated by this method has helped to reveal genetic diversity, population structure and transmission of many microbial pathogens. Advances in DNA sequencing technologies have made it possible to identify microorganisms by multilocus sequencing typing...
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