Comparison of pulsed field gel electrophoresis and repetitive sequence polymerase chain reaction as genotyping methods for detection of genetic diversity and inferring transmission of Salmonella.

doi:10.1016/j.vetmic.2004.02.009

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Article

Comparison of pulsed field gel electrophoresis and repetitive sequence polymerase chain reaction as genotyping methods for detection of genetic diversity and inferring transmission of Salmonella.

Veterinary microbiology - 3 Jun 2004

Weigel Ronald M, Qiao Baozhen, Teferedegne Belete, Suh Dong Kyun, Barber David A, Isaacson Richard E, White Bryan A

Abstract excerpt

Pulsed field gel electrophoresis (PFGE) using restriction enzymes AvrII, SpeI, and XbaI, and repetitive sequence polymerase chain reaction (Rep-PCR) using BOX, ERIC, and REP primers, were compared with respect to their ability to detect genetic differences among 68 Salmonella isolates from nine Illinois swine farms. Both genotyping methods had high reproducibility of fragment numbers (reliability>0.9) and sizes...

Topics

Animals
Cluster Analysis
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Genetic Variation
Genotype
Phylogeny
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Reproducibility of Results

Salmonella

Salmonella Infections, Animal

Serotyping

Swine

Swine Diseases

Source

Open in PubMed

Identifiers

PMID: 15145499
DOI: 10.1016/j.vetmic.2004.02.009

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-15145499-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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