Article
Real-Time PCR System for Detection of Orthopoxviruses and Simultaneous Identification of Smallpox Virus
1 May 2004
Abstract excerpt
A screening assay for real-time LightCycler (Roche Applied Science, Mannheim, Germany) PCR identification of smallpox virus DNA was developed and compiled in a kit system under good manufacturing practice conditions with standardized reagents. In search of a sequence region unique to smallpox virus, the nucleotide sequence of the 14-kDa fusion protein gene of each of 14 variola virus isolates of the Russian World...
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