Article
Real-time PCR assay for specific detection of cowpox virus.
Journal of virological methods - 1 Jan 2015
Maksyutov Rinat A, Gavrilova Elena V, Meyer Hermann, Shchelkunov Sergei N
Abstract excerpt
The species cowpox virus (CPXV), genus Orthopoxvirus (OPV), consists of isolates highly variable in their biological properties and their genotypes. A TaqMan PCR assay for the specific detection of CPXV DNA based on sequences of the ORF D11L has been developed recently. (Gavrilova et al., 2010; Shchelkunov et al., 2011); however, a rather limited panel of CPXV stains has been used. When a much larger panel of 47...
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