Back to search

Article

Evaluation and Clinical Validation of Monkeypox Virus Real-Time PCR Assays

2022-11-14

Abstract excerpt

<h4>BACKGROUND</h4> In spring of 2022, an outbreak of monkeypox spread worldwide. Here, we describe performance characteristics of monkeypox virus (MPXV)-specific and pan-orthopoxvirus qPCR assays for clinical use. <h4>METHODS</h4> We validated probe-based qPCR assays targeting MPXV-specific loci F3L and G2R (genes MPXVgp052/OPG065 and MPXVgp002 and gp190/OPG002, respectively) and a pan-orthopoxvirus assay targeti...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
a3390dc9-a6ac-5a09-87f0-cfe2625486d7
DOI
10.1101/2022.11.12.22282254
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Evaluation and Clinical Validation of Monkeypox Virus Real-Time PCR AssaysDOI 10.1101/2022.11.12.22282254
Validation and implementation of an orthopoxvirus qualitative real-time PCR for the diagnosis of monkeypox in the clinical laboratorySemantic proximity
Multi-center evaluation of the Research Use Only NeuMoDx monkeypox virus (MPXV) fully automated real-time PCR assaySemantic proximity
Evaluation and clinical validation of pan-specific and clade-specific diagnostic real-time PCR assays for monkeypox virusSemantic proximity
An in silico analysis of PCR-based monkeypox virus detection assays: a case study for ongoing clinical surveillanceSemantic proximity
Development and Characterization of Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of Monkeypox VirusSemantic proximity
Wide mismatches in the sequences of primers and probes for Monkeypox virus diagnostic assaysSemantic proximity
Wide mismatches in the sequences of primers and probes for monkeypox virus diagnostic assaysSemantic proximity
A comprehensive review of monkeypox virus and mpox characteristicsSemantic proximity
Monkeypox (mpox) virus: Classification, origin, transmission, genome organization, antiviral drugs, and molecular diagnosisSemantic proximity
A Comparative Evaluation of Three Diagnostic Assays for the Detection of Human MonkeypoxSemantic proximity
Monkeypox: Significance of Various Clinical Samples, Clinical Manifestation, and Molecular Genetic Analysis – Experience of BulgariaSemantic proximity
Clinical Manifestation, Transmission, Pathogenesis, and Diagnosis of Monkeypox Virus: A Comprehensive ReviewSemantic proximity
Select a neighboring publication to make it the new centre.