Article
Development of a plant viral-vector-based gene expression assay for the screening of yeast cytochrome p450 monooxygenases.
Assay and drug development technologies - 1 Feb 2003
Hanley Kathleen, Nguyen Long V, Khan Faizah, Pogue Gregory P, Vojdani Fakhrieh, Panda Sanjay, Pinot Franck, Oriedo Vincent B, Rasochova Lada, Subramanian Mani, Miller Barbara, White Earl L
Abstract excerpt
Development of a gene discovery tool for heterologously expressed cytochrome P450 monooxygenases has been inherently difficult. The activity assays are labor-intensive and not amenable to parallel screening. Additionally, biochemical confirmation requires coexpression of a homologous P450 reducta...
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