Article
Molecular cloning and functional expression in yeast of CYP76B1, a xenobiotic-inducible 7-ethoxycoumarin O-de-ethylase from Helianthus tuberosus.
The Plant journal : for cell and molecular biology - 1 Apr 1998
Batard Y, LeRet M, Schalk M, Robineau T, Durst F, Werck-Reichhart D
Abstract excerpt
In order to obtain plant markers of chemical stress and possible tools for the bio-monitoring of pollution, a protein purification/PCR approach was used to isolate cDNAs of xenobiotic-inducible P450 oxygenases. O-dealkylation of 7-ethoxycoumarin is catalysed in Helianthus tuberosus by cytochromes P450 strongly inducible by a wide range of xenobiotics. Therefore, a 7-ethoxycoumarin O-de-ethylase (ECOD) was...
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