Article
Molecular cloning and functional expression in yeast of CYP76B1, a xenobiotic-inducible 7-ethoxycoumarin O-de-ethylase from Helianthus tuberosus.
The Plant journal : for cell and molecular biology - 1 Apr 1998
Batard Y, LeRet M, Schalk M, Robineau T, Durst F, Werck-Reichhart D
Abstract excerpt
In order to obtain plant markers of chemical stress and possible tools for the bio-monitoring of pollution, a protein purification/PCR approach was used to isolate cDNAs of xenobiotic-inducible P450 oxygenases. O-dealkylation of 7-ethoxycoumarin is catalysed in Helianthus tuberosus by cytochromes...
Topics
- 7-Alkoxycoumarin O-Dealkylase
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Cytochrome P-450 Enzyme System
- DNA Primers
- DNA, Complementary
- DNA, Plant
- Enzyme Induction
- Gene Expression
- Helianthus
- Molecular Sequence Data
- Phylogeny
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid
- Xenobiotics