Article
Changes in the ratio of type-I and type-II collagen expression during monolayer culture of human chondrocytes.
The Journal of bone and joint surgery. British volume - 1 Mar 2004
Marlovits S, Hombauer M, Truppe M, Vècsei V, Schlegel W
Abstract excerpt
We compared the changes in the ratio of type-I and type-II collagen in monolayer cultures of human articular chondrocytes (HAC). HAC were isolated from samples of cartilage from normal joints and cultivated in monolayer for up to 46 days. Expression of collagen type-I and type-II was determined by immunocytochemistry, Western blotting, and the nested reverse transcription polymerase chain reaction (RT-PCR), and...
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