Article
Rapid chromosomal assignment of medaka mutants by bulked segregant analysis.
Gene - 31 Mar 2004
Martinez-Morales Juan-Ramon, Naruse Kiyoshi, Mitani Hiroshi, Shima Akihiro, Wittbrodt Joachim
Abstract excerpt
Genetic screens in medaka are leading to the identification of an increasing number of unique mutant phenotypes. However, so far only a few genes responsible for these phenotypes have been characterized. Furthermore, no protocols using a systematic positional cloning strategy have been developed to determine the implicated genes. The PCR-based bulked segregant analysis is a fast and reliable tool to accomplish...
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