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Anchoring 9,371 maize expressed sequence tagged unigenes to the bacterial artificial chromosome contig map by two-dimensional overgo hybridization.

Plant physiology - 1 Apr 2004

Gardiner Jack, Schroeder Steven, Polacco Mary L, Sanchez-Villeda Hector, Fang Zhiwei, Morgante Michele, Landewe Tim, Fengler Kevin, Useche Francisco, Hanafey Michael, Tingey Scott, Chou Hugh, Wing Rod, Soderlund Carol, Coe Edward H

Abstract excerpt

Our goal is to construct a robust physical map for maize (Zea mays) comprehensively integrated with the genetic map. We have used a two-dimensional 24 x 24 overgo pooling strategy to anchor maize expressed sequence tagged (EST) unigenes to 165,888 bacterial artificial chromosomes (BACs) on high-density filters. A set of 70,716 public maize ESTs seeded derivation of 10,723 EST unigene assemblies. From these...

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Share to Chromosomes, Artificial, Bacterial
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Polymorphism, Single Nucleotide

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Source

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Identifiers

PMID: 15020742
DOI: 10.1104/pp.103.034538
PMCID: PMC419808

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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