Quantitative Adenovirus Neutralization Assays Based on the Secreted Alkaline Phosphatase Reporter Gene: Application in Epidemiologic Studies and in the Design of Adenovector Vaccines

doi:10.1089/104303404322886147

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Quantitative Adenovirus Neutralization Assays Based on the Secreted Alkaline Phosphatase Reporter Gene: Application in Epidemiologic Studies and in the Design of Adenovector Vaccines

1 Mar 2004

Abstract excerpt

Replication-defective recombinant adenoviruses (rAd) are used as vectors for vaccines as well as for gene therapy. To determine type-specific antibodies to adenovirus (Ad) serotypes 2, 5, 24, 34, and 35, we developed quantitative neutralization assays using recombinant adenoviruses with the secreted alkaline phosphatase (SEAP) reporter gene. Among the standardized parameters, the concentration of infectious and...

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Quantitative Adenovirus Neutralization Assays Based on the Secreted Alkaline Phosphatase Reporter Gene: Application in Epidemiologic Studies and in the Design of Adenovector Vaccines

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