Article
Identification and characterization of Clostridium perfringens using single target DNA microarray chip.
International journal of food microbiology - 15 Mar 2004
Al-Khaldi Sufian F, Villanueva Doralis, Chizhikov Vladimir
Abstract excerpt
A DNA microarray method was developed to identify the presence of toxin genes: encoding beta toxin (cpb), epsilon toxin (etx), enterotoxin (cpe), alpha toxin (cpa), and iota toxin (iA) in Clostridium perfringens. To build the DNA chip, each gene sequence was represented by one approximately 22-bp amino-modified oligonucleotide printed twice on aldehyde-coated slides. Multiplex PCR with Cy3 and Cy5-dCTP...
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