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Article

Simultaneous detection of seven mutations with seven forward primers and one common reverse primer in a single PCR step.

Journal of biochemical and biophysical methods - 27 Feb 2004

Wang Qiang, Liu Qiuyun, Li Bao-jian

Abstract excerpt

A new PCR method is described here to amplify simultaneously several fragments sharing the same template in a single PCR step with a hemi-primer. Using this method, we successfully detected various point mutations in tissue plasminogen activator (tPA) mutants.

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Identifiers

PMID: 14980788
DOI: 10.1016/j.jbbm.2003.10.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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