Article
Characterization of the anti-BP180 autoantibody reactivity profile and epitope mapping in bullous pemphigoid patients.
The Journal of investigative dermatology - 1 Jan 2004
Di Zenzo Giovanni, Grosso Fabiana, Terracina Michela, Mariotti Feliciana, De Pità Ornella, Owaribe Katsushi, Mastrogiacomo Alessandro, Sera Francesco, Borradori Luca, Zambruno Giovanna
Abstract excerpt
Bullous pemphigoid is a subepidermal bullous disease of skin and mucosae associated with autoantibodies to BP180. To characterize the humoral response to BP180, we generated a random BP180 epitope library displayed on lambda bacteriophage. After validation of the library by epitope mapping of three BP180-specific monoclonal antibodies, 15 novel or known BP180 epitopes were identified using 10 bullous pemphigoid...
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