Proteolytic processing of secretory proteins in Paramecium: immunological and biochemical characterization of the precursors of trichocyst matrix proteins.

Abstract excerpt

We used polyclonal serum raised against mature trichocyst matrix proteins to detect their unprocessed precursors, a group of proteins (45-55 kDa) present in the whole-cell extract. These precursor proteins were partially purified from the soluble fraction of wild-type cells by ammonium sulfate pr...

Topics

• Animals
• Antibodies, Monoclonal
• Calcium
• Cytoplasmic Granules
• Microscopy, Immunoelectron
• Molecular Weight
• Mutation
• Oxidation-Reduction
• Paramecium
• Protein Conformation
• Protein Precursors
• Protein Processing, Post-Translational