Article
Sequence-specific gene silencing in murine muscle induced by electroporation-mediated transfer of short interfering RNA.
The journal of gene medicine - 1 Jan 2004
Kishida Tsunao, Asada Hidetsugu, Gojo Satoshi, Ohashi Suzuyo, Shin-Ya Masaharu, Yasutomi Kakei, Terauchi Ryu, Takahashi Kenji A, Kubo Toshikazu, Imanishi Jiro, Mazda Osam
Abstract excerpt
BACKGROUND: Post-genomic biomedical research requires efficient techniques for functional analyses of poorly characterized genes in living organisms. Sequence-specific gene silencing in mammalian organs may provide valuable information on the physiological and pathological roles of predicted genes in mammalian systems. Here, we attempted targeted gene knockdown in vivo in murine skeletal muscle through the...
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