Article
Detection of p53 gene mutations in the supernatant of pancreatic juice and plasma from patients with pancreatic carcinomas.
Pancreas - 1 Jan 2004
Wang Ying, Yamaguchi Yasushi, Watanabe Hiroyuki, Ohtsubo Koushirou, Motoo Yoshiharu, Sawabu Norio
Abstract excerpt
AIM: The sensitivity of pure pancreatic juice (PPJ) cytology for the diagnosis of pancreatic carcinoma (PCa) is still low. The usefulness of genetic analyses of PPJ seems to be limited because of insufficient sensitivity or false positivity. To improve the molecular diagnosis of PCa, we analyzed mutations of p53 together with K-ras in DNA extracted not only from the sediment but also from the supernatant of PPJ...
Topics
- Adult
- Aged
- Aged, 80 and over
- Base Sequence
- DNA Mutational Analysis
- DNA, Neoplasm
- Female
- Genes, ras
- Humans
- Male
- Middle Aged
- Mutation
- Pancreatic Juice
- Pancreatic Neoplasms
- Polymorphism, Single-Stranded Conformational