Article
Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway.
Journal of bacteriology - 1 Jan 2004
DeLisa Matthew P, Lee Philip, Palmer Tracy, Georgiou George
Abstract excerpt
Overexpression of either heterologous or homologous proteins that are routed to the periplasm via the twin-arginine translocation (Tat) pathway results in a block of export and concomitant accumulation of the respective protein precursor in the cytoplasm. Screening of a plasmid-encoded genomic library for mutants that confer enhanced export of a TorA signal sequence (ssTorA)-GFP-SsrA fusion protein, and thus...
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