Article
The effect of pfl gene knockout on the metabolism for optically pure D-lactate production by Escherichia coli.
Applied microbiology and biotechnology - 1 Apr 2004
Zhu J, Shimizu K
Abstract excerpt
The effect of gene knockout on metabolism in the pflA-, pflB-, pflC-, and pflD- mutants of Escherichia coli was investigated. Batch cultivations of the pfl- mutants and their parent strain were conducted using glucose as a carbon source. It was found that pflA- and pflB- mutants, but not pflC- and pflD- mutants, produced large amounts of D-lactate from glucose under the microaerobic condition, and the maximum...
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