Article
Inhibition studies on Rhodospirillum rubrum H(+)-pyrophosphatase expressed in Escherichia coli.
Biochimica et biophysica acta - 7 Jun 2004
Schultz Anders, Baltscheffsky Margareta
Abstract excerpt
The membrane-bound proton-pumping inorganic pyrophosphatase from Rhodospirillum rubrum was heterologously expressed in Escherichia coli C43(DE3) cells and was inhibited by 4-bromophenacyl bromide (BPB), N,N'-dicyclohexylcarbodiimid (DCCD), diethyl pyrocarbonate (DEPC) and fluorescein 5'-isothiocyanate (FITC). In each case, the enzyme activity was rather well protected against inhibitory action by the substrate...
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