Article
Rhodospirillum rubrum has a family I pyrophosphatase: purification, cloning, and sequencing.
Archives of microbiology - 1 May 2003
Romero Irma, García-Contreras Rodolfo, Celis Heliodoro
Abstract excerpt
The cytoplasmic pyrophosphatase of the photosynthetic bacterium Rhodospirillum rubrum was purified to electrophoretic homogeneity. The enzyme is a homohexamer of 20-kDa monomers. The gene was cloned and sequenced. Alignment of the deduced 179-amino-acid protein with known bacterial pyrophosphatases revealed conservation of all residues in the active site. Attempts to obtain an insertion mutant of the cytoplasmic...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
