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Detection of Infectious Adenovirus in Cell Culture bymRNA ReverseTranscription-PCR

1 Dec 2003

Abstract excerpt

We have developed and evaluated the reverse transcription (RT)-PCR detection of mRNA in cell culture to assay infectious adenoviruses (Ads) by using Ad type 2 (Ad2) and Ad41 as models. Only infectious Ads are detected because they are the only ones able to produce mRNA during replication in cell culture. Three primer sets for RT-PCR amplification of mRNA were evaluated for their sensitivity and specificity: a...

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PMID: 14660388
DOI: 10.1128/aem.69.12.7377-7384.2003

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