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Quantitative Detection and Rapid Identification of Human Adenoviruses

18 Jan 2007

Abstract excerpt

We have established a method of quantitative detection and rapid identification of human adenoviruses (hAdVs). Using LightCycler PCR with a primer set, we were able to amplify 554 bp of the hexon gene from each of 51 prototype strains of hAdVs. The sensitivity of LightCycler PCR was 10 copies of hAdV DNA/reaction. When LightCycler PCR was performed using a set of primers, hAdV was positive for 74.4% (99 of 133)...

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PMID: 17229856
DOI: 10.1128/jcm.01603-06

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