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Article

Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

19 Jan 2021

Abstract excerpt

We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.

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Identifiers

PMID: 33496233
DOI: 10.3201/eid2702.204168

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