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Rapid and quantitative detection of human adenovirus DNA by real‐time PCR

9 Apr 2003

Abstract excerpt

Rapid diagnosis of human adenovirus (HAdV) infections was achieved by PCR in the recent years. However, conventional PCR has the risk of carry-over contamination due to open handling with its products, and results are only qualitative. Therefore, a quantitative "real-time" PCR with consensus primer and probe (dual fluorescence labelled, "TaqMan") sequences for a conserved region of the hexon gene was designed and...

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PMID: 12696109
DOI: 10.1002/jmv.10382

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