Article
Detection of α-thalassemia-1 Southeast Asian and Thai type deletions and β-thalassemia 3.5-kb deletion by single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting analysis.
The Korean journal of laboratory medicine - 1 Jul 2011
Pornprasert Sakorn, Wiengkum Thanatcha, Srithep Sarinee, Chainoi Isarapong, Singboottra Panthong, Wongwiwatthananukit Sanchai
Abstract excerpt
BACKGROUND: Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia. METHODS: Single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting (HRM) analysis were used for the identification of α-thalassemia-1 Southeast Asian (SEA) and Thai type deletions and β-thalassemia 3.5-kb...
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