Article
VNTR polymorphism of the collagen type II, alpha 1 (COL2A1) gene detected by PCR.
Nihon hoigaku zasshi = The Japanese journal of legal medicine - 1 Oct 1992
Katsuura Y, Maeiwa M
Abstract excerpt
The 3' side of the human type II collagen alpha 1 (COL2A1) gene contains a region consisting of a variable number of tandemly repeated short A + T-rich DNA sequences (VNTR). We amplified this region accurately by the polymerase chain reaction (PCR). Genomic DNA was purified from isolated buffy-coat cells, and thermostable Taq polymerase was used to amplify the target region. The amplification products were...
Topics
- Alleles
- Asian People
- Collagen
- DNA
- Gene Expression Regulation
- Gene Frequency
- Genome, Human
- Heterozygote
- Humans
- Japan
- Polymerase Chain Reaction
- Polymorphism, Genetic
