Article
Site-directed mutation makes rabbit calcyclin dimer.
FEBS letters - 14 Dec 1992
Ando Y, Watanabe M, Akatsuka H, Tokumitsu H, Hidaka H
Abstract excerpt
Unlike human, rat and mouse calcyclin, purified rabbit calcyclin did not form a dimer on Tricine SDS-PAGE under non-reduced conditions. Based on the internal peptide sequence of rabbit calcylin, we isolated and sequenced a cDNA clone encoding calcyclin. The sequence of this clone (pCalC) is 629 bp long and codes 90 amino acid residues of a protein with a molecular mass of 10,153 Da. By Northern blot analysis, a...
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