Article
Antigenic analysis of nonstructural protein (NSP) 4 of group A avian rotavirus strain PO-13.
Microbiology and immunology - 1 Jan 2003
Borgan Mohammed Ali, Mori Yoshio, Ito Naoto, Sugiyama Makoto, Minamoto Nobuyuki
Abstract excerpt
In order to analyze the antigenic structure of nonstructural protein (NSP) 4 of group A avian rotavirus strain PO-13, 25 monoclonal antibodies (MAbs) against NSP4 expressed in Escherichia coli were produced. All MAbs reacted with NSP4 on Western blotting, indicating that they recognized sequential epitopes. To determine the antigenic sites (ASs) recognized by the produced MAbs, seven truncated NSP4s were...
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