Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity.

doi:10.1023/a:1026007923908

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Article

Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity.

Antonie van Leeuwenhoek - 1 Jan 2003

Ruiz-Herrera José, García-Maceira Patricia, Castillo-Barahona Luis C, Valentín Eulogio, Sentandreu Rafael

Abstract excerpt

A collection of transposon-mutagenized strains of Yarrowia lipolytica was screened for wall defects by determination of their sensitivity to calcofluor white. A number of strains were hypersensitive, whereas others were resistant. Different non-allelic mutants displayed increased sensitivity to autolysis and lytic enzymes, independently of whether they were sensitive or resistant to calcofluor white. A thorough...

Topics

Benzenesulfonates
Cell Fractionation
Cell Wall
Chitin
Chitinases
Culture Media
DNA Transposable Elements
Drug Resistance, Fungal
Fungal Proteins
Hydrolases
Mutagenesis, Insertional
Mutation
Yarrowia

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Open in PubMed

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PMID: 14574118
DOI: 10.1023/a:1026007923908

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