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Formation of glucosylceramide and sterol glucoside by a UDP-glucose-dependent glucosylceramide synthase from cotton expressed in Pichia pastoris.

FEBS letters - 23 Oct 2003

Hillig Inga, Leipelt Martina, Ott Claudia, Zähringer Ulrich, Warnecke Dirk, Heinz Ernst

Abstract excerpt

In plants, glucosylceramide (GlcCer) biosynthesis is poorly understood. Previous investigations suggested that sterol glucoside (SG) acts as the actual glucose donor for the plant GlcCer synthase (GCS). We addressed this question by generating a Pichia pastoris double mutant devoid of GlcCer and SG. This mutant was used for heterologous expression of the plant GCS. The activity of the GCS resulted in the...

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PMID: 14572652
DOI: 10.1016/s0014-5793(03)01058-5

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