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Detection of Infectious Bronchitis Virus by Real-Time Reverse Transcriptase–Polymerase Chain Reaction and Identification of a Quasispecies in the Beaudette Strain

1 Jul 2003

Abstract excerpt

In this report, we describe a real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) diagnostic test for infectious bronchitis virus (IBV) with the use of fluorescence resonance energy transfer (FRET) technology. Two primers that amplify a 383-base pair product between nucleotide positions 703 and 1086 relative to the start codon for the S1 gene of the Massachusetts 41 virus were designed and used to...

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PMID: 14562902
DOI: 10.1637/6075

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