Article
Identification of infectious bursal disease virus quasispecies in commercial vaccines and field isolates of this double-stranded RNA virus.
Virology - 5 Dec 2002
Jackwood Daral J, Sommer Susan E
Abstract excerpt
Quasispecies of infectious bursal disease virus (IBDV) vaccine and wild-type strains were identified using real-time RT-PCR at a region of the viral genome known for sequence variability. The LightCycler (Idaho Technology, Inc.) and hybridization probe system (Roche, Molecular Biochemicals) were used. An anchor probe labeled with LightCycler Red 640 and mutation probe labeled with fluorescein were designed using...
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