Article
Statistical approaches for estimating actinobacterial diversity in marine sediments.
Applied and environmental microbiology - 1 Oct 2003
Stach James E M, Maldonado Luis A, Masson Douglas G, Ward Alan C, Goodfellow Michael, Bull Alan T
Abstract excerpt
Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with >/= 99% 16S rDNA sequence similarity; the cutoff value for an OTU was derived by comparing 16S...
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