Article
Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing.
Journal of bacteriology - 1 Jul 1991
Schmidt T M, DeLong E F, Pace N R
Abstract excerpt
The phylogenetic diversity of an oligotrophic marine picoplankton community was examined by analyzing the sequences of cloned ribosomal genes. This strategy does not rely on cultivation of the resident microorganisms. Bulk genomic DNA was isolated from picoplankton collected in the north central Pacific Ocean by tangential flow filtration. The mixed-population DNA was fragmented, size fractionated, and cloned...
Topics
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Ribosomal
- Genetic Variation
- Molecular Sequence Data
- Oligonucleotide Probes
- Phylogeny
- Plankton
- Polymerase Chain Reaction
- RNA, Ribosomal, 16S
