Article
DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities.
FEMS microbiology letters - 15 Dec 1992
Rochelle P A, Fry J C, Parkes R J, Weightman A J
Abstract excerpt
A protocol was devised which permitted the extraction of DNA from deep marine sediments up to 503 m below the sea floor. These sediments have been laid down over the last 3 million years. 16S rRNA gene sequences were amplified from the DNA by the polymerase chain reaction. The details of the successful extraction and polymerase chain reaction methodology varied between samples from different depths. This...
Topics
- Bacteria
- Base Sequence
- DNA, Bacterial
- Genetic Variation
- Marine Biology
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Bacterial
- RNA, Ribosomal, 16S
- Water Microbiology