Article
Novel Plasmid-Borne Fimbriae-Associated Gene Cluster Participates in Biofilm Formation in Escherichia coli.
Microbial drug resistance (Larchmont, N.Y.) - 1 Dec 2021
He Yu-Zhang, Xu Ying, Sun Jian, Gao Bei-Le, Li Gong, Zhou Yu-Feng, Lian Xin-Lei, Fang Liang-Xing, Liao Xiao-Ping, Mediavilla Jose R, Chen Liang, Liu Ya-Hong
Abstract excerpt
This study reported the involvement of a gene cluster from a conjugative plasmid in the biofilm formation of Escherichia coli. We used a novel EZ-Tn5 transposon technique to generate a transposon library and used arbitrarily primed PCR to detect the insertion sites in biofilm formation-deficient mutants. To validate the function of candidate biofilm formation genes, the genes were cloned into plasmid pBluescript...
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