Article
hsp65 sequencing for identification of rapidly growing mycobacteria.
Journal of clinical microbiology - 1 Mar 1999
Ringuet H, Akoua-Koffi C, Honore S, Varnerot A, Vincent V, Berche P, Gaillard J L, Pierre-Audigier C
Abstract excerpt
Partial sequencing of the hsp65 gene was used for the identification of rapidly growing mycobacteria (RGM). A 441-bp fragment (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was amplified and sequenced by an automated fluorescence-based method involving capillary electrophoresis. Type strains of 10 RGM species were first studied. Each species had a...
Topics
- Antigens, Bacterial
- Bacterial Proteins
- Base Sequence
- Chaperonin 60
- Chaperonins
- Genetic Variation
- Humans
- Molecular Sequence Data
- Mycobacterium
- Mycobacterium Infections
- Mycobacterium tuberculosis
- Phylogeny
- Polymerase Chain Reaction
- RNA, Bacterial