Article
The role of FhuD2 in iron(III)-hydroxamate transport in Staphylococcus aureus. Demonstration that FhuD2 binds iron(III)-hydroxamates but with minimal conformational change and implication of mutations on transport.
The Journal of biological chemistry - 12 Dec 2003
Sebulsky M Tom, Shilton Brian H, Speziali Craig D, Heinrichs David E
Abstract excerpt
The fhuD2 gene encodes a lipoprotein that has previously been shown to be important for the utilization of iron(III)-hydroxamates by Staphylococcus aureus. We have studied the function of the FhuD2 protein in greater detail, and demonstrate here that the protein binds several iron(III)-hydroxamates. Mutagenesis of FhuD2 identified several residues that were important for the ability of the protein to function in...
Topics
- Amino Acid Sequence
- Biological Assay
- Biological Transport
- Carrier Proteins
- Conserved Sequence
- Escherichia coli
- Escherichia coli Proteins
- Ferric Compounds
- Glutamic Acid
- Hydroxamic Acids