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Prognostic significance of quantitative analysis of <i>WT1</i> gene transcripts by competitive reverse transcription polymerase chain reaction in acute leukaemia

26 Sept 2003

Abstract excerpt

We have developed a sensitive, competitive, nested reverse transcription polymerase chain reaction (RT-PCR) titration assay that quantifies the number of Wilm's tumour (WT1) gene transcripts in bone marrow (BM) and peripheral blood (PB), coupled with a competitive RT-PCR protocol for the ABL gene as control. We studied BM/PB samples from 107 acute myeloid leukaemia (AML) patients and 22 acute lymphoblastic...

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PMID: 14510942
DOI: 10.1046/j.1365-2141.2003.04552.x

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