Article
A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach.
Nucleic acids research - 1 Oct 2003
Flavell Andrew J, Bolshakov Viacheslav N, Booth Allan, Jing Runchun, Russell Joanne, Ellis T H Noel, Isaac Peter
Abstract excerpt
A microarray-based method has been developed for scoring thousands of DNAs for a co-dominant molecular marker on a glass slide. The approach was developed to detect insertional polymorphism of transposons and works well with single nucleotide polymorphism (SNP) markers. Biotin- terminated allele-specific PCR products are spotted unpurified onto streptavidin-coated glass slides and visualised by hybridisation of...
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