Article
Rapid detection of Flt3 mutations in acute myeloid leukemia patients by denaturing HPLC.
Clinical chemistry - 1 Oct 2003
Bianchini Michele, Ottaviani Emanuela, Grafone Tiziana, Giannini Barbara, Soverini Simona, Terragna Carolina, Amabile Marilina, Piccaluga Pier Paolo, Malagola Michele, Rondoni Michela, Bosi Costanza, Baccarani Michele, Martinelli Giovanni
Abstract excerpt
BACKGROUND: fms-related tyrosine kinase 3 (Flt3) is the most commonly mutated gene in human acute myeloid leukemia (AML) and has been implicated in its pathogenesis. Because screening of Flt3 in AML patients by PCR followed by gel electrophoresis is time-consuming and fails to detect some very small internal tandem duplications (ITDs), we developed a method for screening of FLT3 receptor mutations with PCR plus...
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