Expression of subunit II of chloroplast H(+)-ATPase in an Escherichia coli mutant lacking subunit b of its H(+)-ATPase.

Abstract excerpt

The DNA of subunit II of the H(+)-ATPase from spinach chloroplast was expressed in Escherichia coli. It was found that a high gene dose is lethal to E. coli. With a lower gene dose subunit II was not able to substitute for the homologous subunit b in the E. coli ATP synthase.

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